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human epithelial amnion  (ATCC)


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    ATCC human epithelial amnion
    Human Epithelial Amnion, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 16365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+epithelial+amnion/HeLa/10__1039_slash_d3nj02910g-120-15-22
    Average 99 stars, based on 16365 article reviews
    human epithelial amnion - by Bioz Stars, 2026-09
    99/100 stars

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    MTT Assay:

    Article Title: Synthesis, characterization, biological evaluation, DFT and molecular docking studies of (<i>Z</i>)-2-((2-bromo-4-chlorophenyl)imino)methyl)-4-chlorophenol and its Co(<scp>ii</scp>), Ni(<scp>ii</scp>), Cu(<scp>ii</scp>), and Zn(<scp>ii</scp>) complexes
    Article Snippet: .. Toxicity effect of the complexes was evaluated on normal human cell lines comprises of (WISHATCC-CCL-25, human epithelial amnion (liver cell lines), and MRC-5-ATCC-CCL-171, human lung fibroblast cell lines), using MTT method.36,37 Samples concentrations of 50 and 100 mM were used against these cell lines. ..



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    Establishment of septic mouse model and the effects of human amnion <t>epithelial</t> cells (hAECs) on cecal ligation and puncture (CLP) mice. (A) Critical steps in the CLP operation in mice. First, the abdominal area was disinfected after shaving (a), the midline skin was incised (b), and the cecum was exposed (c). High-grade sepsis was induced by ligation of 75% of the cecum (d). Then, cecal puncture (through and through) was performed using 21-gauge needle (e). After removing the needle (f), the wound was closed (g) and the abdominal skin was sutured (h). (B) Survival rate after induction of sepsis by CLP in mice. ** P < 0.01 vs. the CLP + vehicle group. (C) Assessment of the clinical signs of mice 16 h after CLP. *** P < 0.001 vs. the sham group, ### P < 0.001 vs. the CLP + vehicle group.
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    Establishment of septic mouse model and the effects of human amnion epithelial cells (hAECs) on cecal ligation and puncture (CLP) mice. (A) Critical steps in the CLP operation in mice. First, the abdominal area was disinfected after shaving (a), the midline skin was incised (b), and the cecum was exposed (c). High-grade sepsis was induced by ligation of 75% of the cecum (d). Then, cecal puncture (through and through) was performed using 21-gauge needle (e). After removing the needle (f), the wound was closed (g) and the abdominal skin was sutured (h). (B) Survival rate after induction of sepsis by CLP in mice. ** P < 0.01 vs. the CLP + vehicle group. (C) Assessment of the clinical signs of mice 16 h after CLP. *** P < 0.001 vs. the sham group, ### P < 0.001 vs. the CLP + vehicle group.

    Journal: Frontiers in Medicine

    Article Title: Human Amnion Epithelial Cells and Their Derived Exosomes Alleviate Sepsis-Associated Acute Kidney Injury via Mitigating Endothelial Dysfunction

    doi: 10.3389/fmed.2022.829606

    Figure Lengend Snippet: Establishment of septic mouse model and the effects of human amnion epithelial cells (hAECs) on cecal ligation and puncture (CLP) mice. (A) Critical steps in the CLP operation in mice. First, the abdominal area was disinfected after shaving (a), the midline skin was incised (b), and the cecum was exposed (c). High-grade sepsis was induced by ligation of 75% of the cecum (d). Then, cecal puncture (through and through) was performed using 21-gauge needle (e). After removing the needle (f), the wound was closed (g) and the abdominal skin was sutured (h). (B) Survival rate after induction of sepsis by CLP in mice. ** P < 0.01 vs. the CLP + vehicle group. (C) Assessment of the clinical signs of mice 16 h after CLP. *** P < 0.001 vs. the sham group, ### P < 0.001 vs. the CLP + vehicle group.

    Article Snippet: Human amnion epithelial cells used in this study were provided by Shanghai iCELL Biotechnology Corporation Ltd. (Shanghai, China).

    Techniques: Ligation

    hAECs ameliorated multiple organ damage of CLP mice. (A) Serum creatinine, alanine aminotransferase, and aspartate aminotransferase concentrations at different times in mice with CLP operation followed by vehicle ( n = 5) or hAECs ( n = 5) injection. * P < 0.05 vs. the CLP + vehicle group. (B) Renal pathology of septic mice at 16 h after CLP or CLP with hAECs treatment. Representative micrographs from each group are shown. The arrows indicate tubular epithelial vacuolization and the loss of brush border and the asterisks indicate Bowman’s capsule expansion (H&E staining; scale bar = 50 μm). The lesions in the kidney were alleviated by the hAECs treatment. (C) Pathology of liver, heart, and lung of septic mice at 16 h after CLP or CLP with hAECs treatment. Representative micrographs from each group are shown. The blue arrows indicate vacuolization of hepatocyte cytoplasm and the blue arrowheads indicate sinusoidal congestion of liver tissues. The red arrows indicate edema and vacuolization of myocardial cells of heart tissues. The black arrows indicate alveolar congestion and hemorrhage. The black arrowheads indicate infiltration or aggregation of neutrophils in airspaces of lung tissues (H&E staining; scale bar = 50 μm). The lesions in the liver, heart, and lung were alleviated by the hAECs treatment. (D) Organ pathological scores representing the degree of lesion damage at 16 h after CLP. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. the sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the CLP + vehicle group.

    Journal: Frontiers in Medicine

    Article Title: Human Amnion Epithelial Cells and Their Derived Exosomes Alleviate Sepsis-Associated Acute Kidney Injury via Mitigating Endothelial Dysfunction

    doi: 10.3389/fmed.2022.829606

    Figure Lengend Snippet: hAECs ameliorated multiple organ damage of CLP mice. (A) Serum creatinine, alanine aminotransferase, and aspartate aminotransferase concentrations at different times in mice with CLP operation followed by vehicle ( n = 5) or hAECs ( n = 5) injection. * P < 0.05 vs. the CLP + vehicle group. (B) Renal pathology of septic mice at 16 h after CLP or CLP with hAECs treatment. Representative micrographs from each group are shown. The arrows indicate tubular epithelial vacuolization and the loss of brush border and the asterisks indicate Bowman’s capsule expansion (H&E staining; scale bar = 50 μm). The lesions in the kidney were alleviated by the hAECs treatment. (C) Pathology of liver, heart, and lung of septic mice at 16 h after CLP or CLP with hAECs treatment. Representative micrographs from each group are shown. The blue arrows indicate vacuolization of hepatocyte cytoplasm and the blue arrowheads indicate sinusoidal congestion of liver tissues. The red arrows indicate edema and vacuolization of myocardial cells of heart tissues. The black arrows indicate alveolar congestion and hemorrhage. The black arrowheads indicate infiltration or aggregation of neutrophils in airspaces of lung tissues (H&E staining; scale bar = 50 μm). The lesions in the liver, heart, and lung were alleviated by the hAECs treatment. (D) Organ pathological scores representing the degree of lesion damage at 16 h after CLP. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. the sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the CLP + vehicle group.

    Article Snippet: Human amnion epithelial cells used in this study were provided by Shanghai iCELL Biotechnology Corporation Ltd. (Shanghai, China).

    Techniques: Injection, Staining

    hAECs-derived exosomes (EXOs) reduced the mortality of septic mice and ameliorated kidney damage. (A) EXOs reduced the mortality of septic mice. * P < 0.05 vs. the CLP + PBS group. (B) Assessment of the clinical signs of mice at 16 h after CLP. *** P < 0.001 vs. the sham group; ### P < 0.001 vs. the CLP + phosphate-buffered saline (PBS) group. (C) Serum creatinine concentration in mice 16 h after CLP with PBS ( n = 5) or EXOs ( n = 5) injection. ** P < 0.01 vs. the sham group; # P < 0.05 vs. the CLP + PBS group. (D) Renal pathology of septic mice kidney at 16 h after CLP or CLP with EXOs treatment and renal pathological scoring. Representative micrographs from each group are shown. The arrows indicate tubular epithelial vacuolization and the loss of brush border and the asterisks indicate Bowman’s capsule expansion (H&E staining; scale bar = 50 μm). The lesions in the kidney were alleviated by the EXOs treatment. *** P < 0.001 vs. the sham group; ### P < 0.001 vs. the CLP + PBS group.

    Journal: Frontiers in Medicine

    Article Title: Human Amnion Epithelial Cells and Their Derived Exosomes Alleviate Sepsis-Associated Acute Kidney Injury via Mitigating Endothelial Dysfunction

    doi: 10.3389/fmed.2022.829606

    Figure Lengend Snippet: hAECs-derived exosomes (EXOs) reduced the mortality of septic mice and ameliorated kidney damage. (A) EXOs reduced the mortality of septic mice. * P < 0.05 vs. the CLP + PBS group. (B) Assessment of the clinical signs of mice at 16 h after CLP. *** P < 0.001 vs. the sham group; ### P < 0.001 vs. the CLP + phosphate-buffered saline (PBS) group. (C) Serum creatinine concentration in mice 16 h after CLP with PBS ( n = 5) or EXOs ( n = 5) injection. ** P < 0.01 vs. the sham group; # P < 0.05 vs. the CLP + PBS group. (D) Renal pathology of septic mice kidney at 16 h after CLP or CLP with EXOs treatment and renal pathological scoring. Representative micrographs from each group are shown. The arrows indicate tubular epithelial vacuolization and the loss of brush border and the asterisks indicate Bowman’s capsule expansion (H&E staining; scale bar = 50 μm). The lesions in the kidney were alleviated by the EXOs treatment. *** P < 0.001 vs. the sham group; ### P < 0.001 vs. the CLP + PBS group.

    Article Snippet: Human amnion epithelial cells used in this study were provided by Shanghai iCELL Biotechnology Corporation Ltd. (Shanghai, China).

    Techniques: Derivative Assay, Saline, Concentration Assay, Injection, Staining